@article {2048,
	title = {Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses.},
	journal = {J Vis Exp},
	year = {2023},
	month = {2023 Sep 11},
	abstract = {<p>A critical challenge in translational research is establishing a viable and efficient interface between patient care in the operating room (OR) and the research laboratory. Here, we developed a protocol for acquiring high-quality liquid biopsies for molecular analyses from the aqueous humor and the vitreous from patients undergoing eye surgery. In this workflow, a Mobile Operating Room Lab Interface (MORLI) cart equipped with a computer, a barcode scanner, and lab instruments, including onboard cold storage, is used to obtain and archive human biological samples. A web-based data privacy-compliant database enables annotating each sample over its lifetime, and a cartesian coordinate system allows tracking each barcoded specimen in storage, enabling quick and accurate retrieval of samples for downstream analyses. Molecular characterization of human tissue samples not only serves as a diagnostic tool (e.g., to distinguish between infectious endophthalmitis and other non-infectious intraocular inflammation) but also represents an important component of translational research, allowing the identification of new drug targets, development of new diagnostic tools, and personalized therapeutics.</p>
},
	issn = {1940-087X},
	doi = {10.3791/65804},
	author = {Wolf, Julian and Chemudupati, Teja and Kumar, Aarushi and Rasmussen, Ditte K and Wai, Karen M and Chang, Robert T and Montague, Artis A and Tang, Peter H and Bassuk, Alexander G and Dufour, Antoine and Mruthrunjaya, Prithvi and Mahajan, Vinit B}
}
