@article {1856, title = {The Present and Future of Mitochondrial-Based Therapeutics for Eye Disease.}, journal = {Transl Vis Sci Technol}, volume = {10}, year = {2021}, month = {2021 07 01}, pages = {4}, abstract = {

Translational Relevance: Mitochondria are viable therapeutic targets for a broad spectrum of ocular diseases.

}, keywords = {Eye Diseases, Humans, Mitochondria}, issn = {2164-2591}, doi = {10.1167/tvst.10.8.4}, author = {Ji, Marco H and Kreymerman, Alexander and Belle, Kinsley and Ghiam, Benjamin K and Muscat, Stephanie P and Mahajan, Vinit B and Enns, Gregory M and Mercola, Mark and Wood, Edward H} } @article {129, title = {Proteomic analysis of vitreous biopsy techniques.}, journal = {Retina}, volume = {32}, year = {2012}, month = {2012 Nov-Dec}, pages = {2141-9}, abstract = {

PURPOSE: To compare vitreous biopsy methods using analysis platforms used in proteomics biomarker discovery.

METHODS: Vitreous biopsies from 10 eyes were collected sequentially using a 23-gauge needle and a 23-gauge vitreous cutter instrument. Paired specimens were evaluated by UV absorbance spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and liquid chromatography tandem mass spectrometry (LC-MS/MS).

RESULTS: The total protein concentration obtained with a needle and vitrectomy instrument biopsy averaged 1.10 mg/mL (standard error of the mean = 0.35) and 1.13 mg/mL (standard error of the mean = 0.25), respectively. In eight eyes with low or medium viscidity, there was a very high correlation (R = 0.934) between the biopsy methods. When data from 2 eyes with high viscidity vitreous were included, the correlation was reduced (R = 0.704). The molecular weight protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of paired needle and vitreous cutter samples were similar, except for a minority of pairs with single band intensity variance. Using LC-MS/MS, equivalent peptides were identified with similar frequencies (R \≥ 0.90) in paired samples.

CONCLUSION: Proteins and peptides collected from vitreous needle biopsies are nearly equivalent to those obtained from a vitreous cutter instrument. This study suggests both techniques may be used for most proteomic and biomarker discovery studies of vitreoretinal diseases, although a minority of proteins and peptides may differ in concentration.

}, keywords = {Adolescent, Aged, Biological Markers, Biopsy, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Eye Diseases, Eye Proteins, Female, Humans, Male, Middle Aged, Proteomics, Spectrophotometry, Ultraviolet, Tandem Mass Spectrometry, Vitrectomy, Vitreous Body, Young Adult}, author = {Skeie, Jessica M and Brown, Eric N and Martinez, Harryl D and Russell, Stephen R and Birkholz, Emily S and Folk, James C and Boldt, H Culver and Gehrs, Karen M and Stone, Edwin M and Wright, Michael E and Mahajan, Vinit B} } @article {165, title = {T-cell infiltration in autosomal dominant neovascular inflammatory vitreoretinopathy.}, journal = {Molecular vision}, volume = {16}, year = {2010}, month = {2010}, pages = {1034-40}, abstract = {

Autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) is a familial blinding disease of unknown pathophysiology. The eyes and sera from patients with ADNIV were studied to understand the immune response in this condition.

}, keywords = {Aged, 80 and over, Antigens, CD4, Antigens, CD8, Autoantibodies, B-Lymphocytes, Eye Diseases, Female, Genes, Dominant, Humans, Immunoglobulin G, Immunologic Techniques, Neovascularization, Pathologic, Retina, Retinal Diseases, Staining and Labeling, T-Lymphocytes, Vitreous Body}, author = {Mahajan, Vinit B and Vallone, John G and Lin, Jonathan H and Mullins, Robert F and Ko, Audrey C and Folk, James C and Stone, Edwin M} }