@article {978, title = {CRISPR GENOME SURGERY IN THE RETINA IN LIGHT OF OFF-TARGETING.}, journal = {Retina}, volume = {38}, year = {2018}, month = {2018 08}, pages = {1443-1455}, abstract = {

PURPOSE: Recent concerns regarding the clinical utilization of clustered regularly interspaced short palindromic repeats (CRISPR) involve uncertainties about the potential detrimental effects that many arise due to unintended genetic changes, as in off-target mutagenesis, during CRISPR genome surgery. This review gives an overview of off-targeting detection methods and CRISPR{\textquoteright}s place in the clinical setting, specifically in the field of ophthalmology.

RESULTS: As CRISPR utilization in the laboratory setting has increased, knowledge regarding CRISPR mechanisms including its off-target effects has also increased. Although a perfect method for achieving 100\% specificity is yet to be determined, the past few years have seen many developments in off-targeting detection and in increasing efficacy of CRISPR tools.

CONCLUSION: The CRISPR system has high potential to be an invaluable therapeutic tool as it has the ability to modify and repair pathogenic retinal lesions. Although it is not yet a perfect system, with further efforts to improve its specificity and efficacy along with careful screening of off-target mutations, CRISPR-mediated genome surgery potential can become maximized and applied to patients.

}, keywords = {Clustered Regularly Interspaced Short Palindromic Repeats, Gene Editing, Genetic Therapy, Humans, Ophthalmology, Retinal Diseases}, issn = {1539-2864}, doi = {10.1097/IAE.0000000000002197}, author = {Cho, Galaxy Y and Schaefer, Kellie A and Bassuk, Alexander G and Tsang, Stephen H and Mahajan, Vinit B} } @article {163, title = {Patients with an acute zonal occult outer retinopathy-like illness rapidly improve with valacyclovir treatment.}, journal = {American journal of ophthalmology}, volume = {150}, year = {2010}, month = {2010 Oct}, pages = {511-8}, abstract = {

To describe 3 cases of an acute zonal occult outer retinopathy-like illness responsive to valacyclovir hydrochloride.

}, keywords = {Acute Disease, Acyclovir, Adolescent, Adult, Antiviral Agents, Electroretinography, Eye Infections, Viral, Female, Herpesviridae Infections, Humans, Male, Retinal Diseases, Retrospective Studies, Scotoma, Tomography, Optical Coherence, Valine, Visual Field Tests, Visual Fields}, author = {Mahajan, Vinit B and Stone, Edwin M} } @article {165, title = {T-cell infiltration in autosomal dominant neovascular inflammatory vitreoretinopathy.}, journal = {Molecular vision}, volume = {16}, year = {2010}, month = {2010}, pages = {1034-40}, abstract = {

Autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) is a familial blinding disease of unknown pathophysiology. The eyes and sera from patients with ADNIV were studied to understand the immune response in this condition.

}, keywords = {Aged, 80 and over, Antigens, CD4, Antigens, CD8, Autoantibodies, B-Lymphocytes, Eye Diseases, Female, Genes, Dominant, Humans, Immunoglobulin G, Immunologic Techniques, Neovascularization, Pathologic, Retina, Retinal Diseases, Staining and Labeling, T-Lymphocytes, Vitreous Body}, author = {Mahajan, Vinit B and Vallone, John G and Lin, Jonathan H and Mullins, Robert F and Ko, Audrey C and Folk, James C and Stone, Edwin M} }