TY - JOUR T1 - A protocol to inject ocular drug implants into mouse eyes. JF - STAR Protoc Y1 - 2022 A1 - Lin, Cheng-Hui A1 - Sun, Young Joo A1 - Lee, Soo Hyeon A1 - Mujica, Elena M A1 - Kunchur, Caitlin R A1 - Wu, Man-Ru A1 - Yang, Jing A1 - Jung, Youn Soo A1 - Chiang, Bryce A1 - Wang, Sui A1 - Mahajan, Vinit B KW - Animals KW - Drug Implants KW - Intravitreal Injections KW - Mice KW - Vitreous Body AB -

Ocular drug implants (ODIs) are beneficial for treating ocular diseases. However, the lack of a robust injection approach for small-eyed model organisms has been a major technical limitation in developing ODIs. Here, we present a cost-effective, minimally invasive protocol to deliver ODIs into the mouse vitreous called Mouse Implant Intravitreal Injection (MI3). MI3 provides two alternative surgical approaches (air-pressure or plunger) to deliver micro-scaled ODIs into milli-scaled eyes, and expands the preclinical platforms to determine ODIs' efficacy, toxicity, and pharmacokinetics. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).

VL - 3 IS - 1 ER -