Title | Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system. |
Publication Type | Journal Article |
Year of Publication | 2022 |
Authors | Wolf, Julian, Sabage Luís E., Sun Young Joo, and Mahajan Vinit B. |
Journal | STAR Protoc |
Volume | 3 |
Issue | 4 |
Pagination | 101754 |
Date Published | 2022 Oct 06 |
ISSN | 2666-1667 |
Abstract | This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissue loss, and scalability. The protocol can be applied to other studies investigating vitreous liquefaction or gelatinous tissue analysis and can also serve to study vitreous liquefaction in vivo as it may occur during aging or disease progression. |
DOI | 10.1016/j.xpro.2022.101754 |
Alternate Journal | STAR Protoc |
PubMed ID | 36208453 |
PubMed Central ID | PMC9562426 |
Grant List | P30 EY026877 / EY / NEI NIH HHS / United States |