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Mouse Microsurgery

The mouse eye is an important genetic model for the translational study of human ophthalmic disease. Blinding diseases in humans, such as macular degeneration, photoreceptor degeneration, cataract, glaucoma, retinoblastoma, and diabetic retinopathy can be recapitulated in transgenic mice. Our team has extensive microsurgical experience with eyes, and we develop and share surgical methods for preclinical research that tests therapies we hope will eventually be used for humans. All studies are conducted in accordance with the ARVO Statement for Use of Animals in Ophthalmic and Vision Research.

Projects

This surgical technique illustrates evisceration of the vitreous, retina, and lens from the mouse eye, separation by centrifugation, and characterization with protein assays

This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.

This surgical technique illustrates removal of the mouse eye for high-thoughput phenotyping.

News

Dec 10 2021 | Posted In: 20/20 Blog
Palo Alto, CA — In the operating room and clinic, Vinit Mahajan M.D., Ph.D., Stanford Ophthalmology vice chair for research and ChEM-H fellow, uses ocular drug implants (ODIs) to safely deliver steroids into the eye to treat a range of eye di
Nov 11 2021 | Posted In: 20/20 Blog
Palo Alto, CA —News outlets, fitness magazines, and health bloggers churn out hundreds of articles on the health benefits of exercise. And most people can tell you that exercise is an important factor in warding off heart disease, diabetes, cancer, and depression.
Sep 30 2021 | Posted In: 20/20 Blog
 
Jun 8 2020 | Posted In: 20/20 Blog, Press
Palo Alto, Ca — Genetic testing doesn’t always provide a diagnosis, so Vinit Mahajan M.D., Ph.D., associate professor of ophthalmology at Stanford University, has applied proteomics to better understand disease in his patients. 
Apr 29 2020 | Posted In: 20/20 Blog
Palo Alto, CA — Traumatic brain injuries (TBI) can happen when someone is close to an explosion that sends out powerful shock waves through the air.

Publications

The Present and Future of Mitochondrial-Based Therapeutics for Eye Disease., Ji, Marco H., Kreymerman Alexander, Belle Kinsley, Ghiam Benjamin K., Muscat Stephanie P., Mahajan Vinit B., Enns Gregory M., Mercola Mark, and Wood Edward H. , Transl Vis Sci Technol, 2021 07 01, Volume 10, Issue 8, p.4, (2021)
Sex Does Not Influence Visual Outcomes After Blast-Mediated Traumatic Brain Injury but IL-1 Pathway Mutations Confer Partial Rescue., Evans, Lucy P., Boehme Nickolas, Wu Shu, Burghardt Elliot L., Akurathi Abhigna, Todd Brittany P., Newell Elizabeth A., Ferguson Polly J., Mahajan Vinit B., Dutca Laura M., et al. , Invest Ophthalmol Vis Sci, 2020 Oct 01, Volume 61, Issue 12, p.7, (2020)
Modulation of Post-Traumatic Immune Response Using the IL-1 Receptor Antagonist Anakinra for Improved Visual Outcomes., Evans, Lucy P., Woll Addison W., Wu Shu, Todd Brittany P., Hehr Nicole, Hedberg-Buenz Adam, Anderson Michael G., Newell Elizabeth A., Ferguson Polly J., Mahajan Vinit B., et al. , J Neurotrauma, 2020 Mar 16, (2020)
Metabolite therapy guided by liquid biopsy proteomics delays retinal neurodegeneration., Wert, Katherine J., Velez Gabriel, Kanchustambham Vijaya L., Shankar Vishnu, Evans Lucy P., Sengillo Jesse D., Zare Richard N., Bassuk Alexander G., Tsang Stephen H., and Mahajan Vinit B. , EBioMedicine, 2020 Feb 03, Volume 52, p.102636, (2020)
Extracellular superoxide dismutase 3 (SOD3) regulates oxidative stress at the vitreoretinal interface, Wert, Katherine J., Velez Gabriel, Cross Madeline R., Wagner Brett A., Teoh-Fitzgerald Melissa L., Buettner Garry R., McAnany Jason, Olivier Alicia, Tsang Stephen H., Harper Matthew M., et al. , Free Radical Biology and Medicine, (2018)
Gene Therapy Restores Mfrp and Corrects Axial Eye Length, Velez, Gabriel, Tsang Stephen H., Tsai Yi-Ting, Hsu Chun-Wei, Gore Anuradha, Abdelhakim Aliaa H., Mahajan Maryann, Silverman Ronald H., Sparrow Janet R., Bassuk Alexander G., et al. , Scientific reports, Volume 7, p.16151, (2017)