|Title||Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses.|
|Publication Type||Journal Article|
|Year of Publication||2023|
|Authors||Wolf, Julian, Chemudupati Teja, Kumar Aarushi, Rasmussen Ditte K., Wai Karen M., Chang Robert T., Montague Artis A., Tang Peter H., Bassuk Alexander G., Dufour Antoine, Mruthrunjaya Prithvi, and Mahajan Vinit B.|
|Journal||J Vis Exp|
|Date Published||2023 Sep 11|
A critical challenge in translational research is establishing a viable and efficient interface between patient care in the operating room (OR) and the research laboratory. Here, we developed a protocol for acquiring high-quality liquid biopsies for molecular analyses from the aqueous humor and the vitreous from patients undergoing eye surgery. In this workflow, a Mobile Operating Room Lab Interface (MORLI) cart equipped with a computer, a barcode scanner, and lab instruments, including onboard cold storage, is used to obtain and archive human biological samples. A web-based data privacy-compliant database enables annotating each sample over its lifetime, and a cartesian coordinate system allows tracking each barcoded specimen in storage, enabling quick and accurate retrieval of samples for downstream analyses. Molecular characterization of human tissue samples not only serves as a diagnostic tool (e.g., to distinguish between infectious endophthalmitis and other non-infectious intraocular inflammation) but also represents an important component of translational research, allowing the identification of new drug targets, development of new diagnostic tools, and personalized therapeutics.
|Alternate Journal||J Vis Exp|