CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology

Authors:

Wu, W. H., Tsai, Y. T., Justus, S., Cho, G. Y., Sengillo, J. D., Xu, Y., Cabral, T., Lin, C. S., Bassuk, A. G., Mahajan, V. B., & Tsang, S. H.

Publication date:

2018-01

Abstract

CRISPR/Cas9 genome engineering is currently the leading genome surgery technology in most genetics laboratories. Combined with other complementary techniques, it serves as a powerful tool for uncovering genotype-phenotype correlations. Here, we describe a simplified protocol that was used in our publication, CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa, providing an overview of each section of the experimental process.

Keywords: CRISPR; Electroretinogram; Fundoscopy; Genome engineering; Optical-coherence tomography; Retinitis pigmentosa; sgRNA construct design.

Citation:

Wu, W. H., Tsai, Y. T., Justus, S., Cho, G. Y., Sengillo, J. D., Xu, Y., ... & Tsang, S. H. (2018). CRISPR repair reveals causative mutation in a preclinical model of retinitis pigmentosa: a brief methodology. Retinal Gene Therapy: Methods and Protocols, 191-205.

PMCID:

PMC9119419

PubMed ID:

29188514

Year of Publication:

2018