Title | An intravitreal implant injection method for sustained drug delivery into mouse eyes. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Sun, Young Joo, Lin Cheng-Hui, Wu Man-Ru, Lee Soo Hyeon, Yang Jing, Kunchur Caitlin R., Mujica Elena M., Chiang Bryce, Jung Youn Soo, Wang Sui, and Mahajan Vinit B. |
Journal | Cell Rep Methods |
Volume | 1 |
Issue | 8 |
Date Published | 2021 Dec 20 |
ISSN | 2667-2375 |
Abstract | Using small molecule drugs to treat eye diseases carries benefits of specificity, scalability, and transportability, but their efficacy is significantly limited by a fast intraocular clearance rate. Ocular drug implants (ODIs) present a compelling means for the slow and sustained release of small molecule drugs inside the eye. However, methods are needed to inject small molecule ODIs into animals with small eyes, such as mice, which are the primary genetic models for most human ocular diseases. Consequently, it has not been possible to fully investigate efficacy and ocular pharmacokinetics of ODIs. Here, we present a robust, cost-effective, and minimally invasive method called "mouse implant intravitreal injection" (MI3) to deliver ODIs into mouse eyes. This method will expand ODI research to cover the breadth of human eye diseases modeled in mice. |
DOI | 10.1016/j.crmeth.2021.100125 |
Alternate Journal | Cell Rep Methods |
PubMed ID | 35128514 |
PubMed Central ID | PMC8813043 |
Grant List | P30 EY026877 / EY / NEI NIH HHS / United States R01 EY024665 / EY / NEI NIH HHS / United States R01 EY026682 / EY / NEI NIH HHS / United States R01 EY024698 / EY / NEI NIH HHS / United States R01 EY025225 / EY / NEI NIH HHS / United States R01 NS109990 / NS / NINDS NIH HHS / United States |