Title | Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Zhang, Jie, Li Liang, Huang Haoliang, Fang Fang, Webber Hannah C., Zhuang Pei, Liu Liang, Dalal Roopa, Tang Peter H., Mahajan Vinit B., Sun Yang, Li Shaohua, Zhang Mingchang, Goldberg Jeffrey L., and Hu Yang |
Journal | Elife |
Volume | 8 |
Date Published | 2019 May 15 |
ISSN | 2050-084X |
Abstract | Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (SO) blocks the pupil or migrates into the anterior chamber following vitreoretinal surgery. We observed significant intraocular pressure (IOP) elevation after intracameral injection of SO, and that SO removal allows IOP to return quickly to normal. This simple, inducible and reversible mouse ocular hypertension model shows dynamic changes of visual function that correlate with progressive retinal ganglion cell (RGC) loss and axon degeneration. It may be applicable with only minor modifications to a range of animal species in which it will generate stable, robust IOP elevation and significant neurodegeneration that will facilitate selection of neuroprotectants and investigating the pathogenesis of ocular hypertension-induced glaucoma. |
DOI | 10.7554/eLife.45881 |
Alternate Journal | Elife |
PubMed ID | 31090540 |
PubMed Central ID | PMC6533060 |
Grant List | T32 EY027816 / EY / NEI NIH HHS / United States NEI T32 EY027816 / / National Eye Institute / EY-25295 / / National Eye Institute / K08-EY022058 / / National Eye Institute / R01 EY023295 / EY / NEI NIH HHS / United States William and Mary Greve Special Scholar Award / / Research to Prevent Blindness / EY027261 / / National Eye Institute / EY023295 / / National Eye Institute / R01 EY026766 / EY / NEI NIH HHS / United States VA CX001298 / / U.S. Department of Veterans Affairs / EY024932 / / National Eye Institute / EY028106 / / National Eye Institute / R01 EY024932 / EY / NEI NIH HHS / United States R01 EY025295 / EY / NEI NIH HHS / United States R01 EY028106 / EY / NEI NIH HHS / United States EY026766 / / National Eye Institute / U01 EY027261 / EY / NEI NIH HHS / United States K08 EY022058 / EY / NEI NIH HHS / United States |