|Title||Proteomic analysis of autoimmune retinopathy implicates NrCAM as a potential biomarker.|
|Publication Type||Journal Article|
|Year of Publication||2022|
|Authors||Al-Moujahed, Ahmad, Velez Gabriel, Vu Jennifer T., de Carvalho Jose R. Lima, Levi Sarah R., Bassuk Alexander G., Sepah Yasir J., Tsang Stephen H., and Mahajan Vinit B.|
|Date Published||2022 Jun|
Purpose: To identify vitreous molecular biomarkers associated with autoimmune retinopathy (AIR).
Design: Case-control study.
Participants: We analyzed six eyes from four patients diagnosed with AIR and eight comparative controls diagnosed with idiopathic macular holes and epiretinal membranes.
Methods: Vitreous biopsies were collected from the participants and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) or multiplex ELISA.
Outcome Measures: Protein expression changes were evaluated by 1-way ANOVA (significant p-value <0.05), hierarchical clustering, and pathway analysis to identify candidate protein biomarkers.
Results: There were 16 significantly upregulated and 17 significantly downregulated proteins in the vitreous of three AIR patients compared to controls. The most significantly upregulated proteins included lysozyme C (LYSC), zinc-alpha-2-glycoprotein (ZA2G), complement factor D (CFAD), transforming growth factor-beta induced protein (BGH3), beta-crystallin B2, and alpha-crystallin A chain. The most significantly downregulated proteins included disco-interacting protein 2 homolog (DIP2C), retbindin (RTBDN), and amyloid beta precursor like protein 2 (APLP2). Pathway analysis revealed that vascular endothelial growth factor (VEGF) signaling was a top represented pathway in the vitreous of AIR patients compared to controls. In comparison to a different cohort of three AIR patients analyzed by multiplex ELISA, a commonly differentially expressed protein was neuronal cell adhesion molecule (NrCAM) with p-values of 0.027 in the LC-MS/MS dataset and 0.035 in the ELISA dataset.
Conclusion: Protein biomarkers such as NrCAM in the vitreous may eventually help diagnose AIR.
|Alternate Journal||Ophthalmol Sci|
|PubMed Central ID||PMC9075676|
|Grant List||P30 EY026877 / EY / NEI NIH HHS / United States |
R01 EY030151 / EY / NEI NIH HHS / United States
R01 EY031952 / EY / NEI NIH HHS / United States
T32 GM139776 / GM / NIGMS NIH HHS / United States