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Proteomic analysis of autoimmune retinopathy implicates NrCAM as a potential biomarker.

TitleProteomic analysis of autoimmune retinopathy implicates NrCAM as a potential biomarker.
Publication TypeJournal Article
Year of Publication2022
AuthorsAl-Moujahed, Ahmad, Velez Gabriel, Vu Jennifer T., de Carvalho Jose R. Lima, Levi Sarah R., Bassuk Alexander G., Sepah Yasir J., Tsang Stephen H., and Mahajan Vinit B.
JournalOphthalmol Sci
Date Published2022 Jun

Purpose: To identify vitreous molecular biomarkers associated with autoimmune retinopathy (AIR).

Design: Case-control study.

Participants: We analyzed six eyes from four patients diagnosed with AIR and eight comparative controls diagnosed with idiopathic macular holes and epiretinal membranes.

Methods: Vitreous biopsies were collected from the participants and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) or multiplex ELISA.

Outcome Measures: Protein expression changes were evaluated by 1-way ANOVA (significant p-value <0.05), hierarchical clustering, and pathway analysis to identify candidate protein biomarkers.

Results: There were 16 significantly upregulated and 17 significantly downregulated proteins in the vitreous of three AIR patients compared to controls. The most significantly upregulated proteins included lysozyme C (LYSC), zinc-alpha-2-glycoprotein (ZA2G), complement factor D (CFAD), transforming growth factor-beta induced protein (BGH3), beta-crystallin B2, and alpha-crystallin A chain. The most significantly downregulated proteins included disco-interacting protein 2 homolog (DIP2C), retbindin (RTBDN), and amyloid beta precursor like protein 2 (APLP2). Pathway analysis revealed that vascular endothelial growth factor (VEGF) signaling was a top represented pathway in the vitreous of AIR patients compared to controls. In comparison to a different cohort of three AIR patients analyzed by multiplex ELISA, a commonly differentially expressed protein was neuronal cell adhesion molecule (NrCAM) with p-values of 0.027 in the LC-MS/MS dataset and 0.035 in the ELISA dataset.

Conclusion: Protein biomarkers such as NrCAM in the vitreous may eventually help diagnose AIR.

Alternate JournalOphthalmol Sci
PubMed ID35529077
PubMed Central IDPMC9075676
Grant ListP30 EY026877 / EY / NEI NIH HHS / United States
R01 EY030151 / EY / NEI NIH HHS / United States
R01 EY031952 / EY / NEI NIH HHS / United States
T32 GM139776 / GM / NIGMS NIH HHS / United States