Title | A protocol to inject ocular drug implants into mouse eyes. |
Publication Type | Journal Article |
Year of Publication | 2022 |
Authors | Lin, Cheng-Hui, Sun Young Joo, Lee Soo Hyeon, Mujica Elena M., Kunchur Caitlin R., Wu Man-Ru, Yang Jing, Jung Youn Soo, Chiang Bryce, Wang Sui, and Mahajan Vinit B. |
Journal | STAR Protoc |
Volume | 3 |
Issue | 1 |
Pagination | 101143 |
Date Published | 2022 03 18 |
ISSN | 2666-1667 |
Keywords | Animals, Drug Implants, Intravitreal Injections, Mice, Vitreous Body |
Abstract | Ocular drug implants (ODIs) are beneficial for treating ocular diseases. However, the lack of a robust injection approach for small-eyed model organisms has been a major technical limitation in developing ODIs. Here, we present a cost-effective, minimally invasive protocol to deliver ODIs into the mouse vitreous called Mouse Implant Intravitreal Injection (MI3). MI3 provides two alternative surgical approaches (air-pressure or plunger) to deliver micro-scaled ODIs into milli-scaled eyes, and expands the preclinical platforms to determine ODIs' efficacy, toxicity, and pharmacokinetics. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021). |
DOI | 10.1016/j.xpro.2022.101143 |
Alternate Journal | STAR Protoc |
PubMed ID | 35141566 |
PubMed Central ID | PMC8810562 |
Grant List | R01 EY024698 / EY / NEI NIH HHS / United States R01 EY026682 / EY / NEI NIH HHS / United States R01 EY025225 / EY / NEI NIH HHS / United States R01 NS109990 / NS / NINDS NIH HHS / United States R01 EY024665 / EY / NEI NIH HHS / United States P30 EY026877 / EY / NEI NIH HHS / United States R01 EY032585 / EY / NEI NIH HHS / United States |