Skip to content Skip to navigation

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice.

TitleA Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice.
Publication TypeJournal Article
Year of Publication2019
AuthorsZhang, Jie, Fang Fang, Li Liang, Huang Haoliang, Webber Hannah C., Sun Yang, Mahajan Vinit B., and Hu Yang
JournalJ Vis Exp
Date Published2019 Nov 15

Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing stable IOP elevation in mice that mimics the post-operative complication of using silicone oil (SO) as a tamponade agent in human vitreoretinal surgery. In this protocol, SO is injected into the anterior chamber of the mouse eye to block the pupil and prevent inflow of aqueous humor. The posterior chamber accumulates aqueous humor and this in turn increases the IOP of the posterior segment. A single SO injection produces reliable, sufficient, and stable IOP elevation, which induces significant glaucomatous neurodegeneration. This model is a true replicate of secondary glaucoma in the eye clinic. To further mimic the clinical setting, SO can be removed from the anterior chamber to reopen the drainage pathway and allow inflow of aqueous humor, which is drained through the trabecular meshwork (TM) at the angle of the anterior chamber. Because IOP quickly returns to normal, the model can be used to test the effect of lowering IOP on glaucomatous retinal ganglion cells. This method is straightforward, does not require special equipment or repeat procedures, closely simulates clinical situations, and may be applicable to diverse animal species. However, minor modifications may be required.

Alternate JournalJ Vis Exp
PubMed ID31789319
PubMed Central IDPMC6938455
Grant ListR01 EY023295 / EY / NEI NIH HHS / United States
R01 EY024932 / EY / NEI NIH HHS / United States
R01 EY028106 / EY / NEI NIH HHS / United States
R21 EY031063 / EY / NEI NIH HHS / United States