|Title||Mouse eye enucleation for remote high-throughput phenotyping.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Mahajan, Vinit B., Skeie Jessica M., Assefnia Amir H., Mahajan Maryann, and Tsang Stephen H.|
|Journal||Journal of visualized experiments : JoVE|
|Keywords||Animals, Eye Enucleation, High-Throughput Screening Assays, Mice, Mice, Transgenic, Phenotype, Tissue Fixation|
The mouse eye is an important genetic model for the translational study of human ophthalmic disease. Blinding diseases in humans, such as macular degeneration, photoreceptor degeneration, cataract, glaucoma, retinoblastoma, and diabetic retinopathy have been recapitulated in transgenic mice.(1-5) Most transgenic and knockout mice have been generated by laboratories to study non-ophthalmic diseases, but genetic conservation between organ systems suggests that many of the same genes may also play a role in ocular development and disease. Hence, these mice represent an important resource for discovering new genotype-phenotype correlations in the eye. Because these mice are scattered across the globe, it is difficult to acquire, maintain, and phenotype them in an efficient, cost-effective manner. Thus, most high-throughput ophthalmic phenotyping screens are restricted to a few locations that require on-site, ophthalmic expertise to examine eyes in live mice. (6-9) An alternative approach developed by our laboratory is a method for remote tissue-acquisition that can be used in large or small-scale surveys of transgenic mouse eyes. Standardized procedures for video-based surgical skill transfer, tissue fixation, and shipping allow any lab to collect whole eyes from mutant animals and send them for molecular and morphological phenotyping. In this video article, we present techniques to enucleate and transfer both unfixed and perfusion fixed mouse eyes for remote phenotyping analyses.
|Alternate Journal||J Vis Exp|