Integrative Structural Biology Lab
Structural biology is used to determine the shape of molecules at atomic resolution. This allows us to understand exactly how disease mutations affect proteins and how we can correct them. An important goal in the lab is to understand the structure of Calpain-5 (CAPN5), a calcium-activated regulatory cysteine protease that is expressed throughout the central nervous system and retina. Mutations in the catalytic core of CAPN5 make it hyperactive and cause Autosomal Dominant Neovascular Inflammatory Vitreoretinopathy (ADNIV; OMIM#193235), a severe, blinding eye disease. Structural studies of CAPN5 ADNIV disease mutants will inform how these mutations alter CAPN5 structure and function. Our laboratory is using x-ray crystallography and other biophysical techniques (small angle x-ray scattering, dynamic light scattering, cryo-EM, and fluorescence assays) to study the structural mechanisms of CAPN5 activation.
The lab also uses molecular modeling to study disease-causing mutations. With the rapidly-reducing costs of whole genome and exome sequencing, clinical genomic testing is being increasingly applied in the identification of pathogenic disease variants in patients. New sequence variants are often identified, but many are classified as variants of uncertain significance (VUS) due to the lack of functional assays to study their effects. Molecular modeling of protein structures can provide information at the atomic level and help to predict the pathogenicity of novel mutations by placing variants of uncertain significance in the context of the patient’s disease, pathophysiology, and protein function. Our team collaborates with clinicians and researchers around the world to model novel disease variants and better understand their patients’ disease. Please contact us for a consultation or collaboration.
Small-angle x-ray scattering (SAXS) is a powerful, alternative tool to x-ray crystallography and can be used to obtain structural information on proteins in the absence of a crystal structure.
Structure-based drug design for inherited eye diseases.
Predicting the effects of disease mutations using structural bioinformatics.
We are using x-ray crystallography to understand the structure of Calpain-5 (CAPN5), a calcium-activated regulatory cysteine protease that is expressed throughout the central nervous system a
Oue research team purifies proteins for crystallography and enzymatic assays at the Macromolecular Structure Knowledge Center (MSKC).
Mar 31 2020 | Posted In: 20/20 Blog
Palo Alto, CA — On an international Zoom conference, Gabriel Velez, an M.D., Ph.D. student in the Mahajan Lab, successfully presented his Ph.D. thesis defense seminar.
Jan 31 2020 | Posted In: 20/20 Blog
Palo Alto, CA — In the article published in the January 2020 issue of Cell Reports, “Structural insight
Nov 4 2019 | Posted In: 20/20 Blog
Cold Spring Harbor, NY — Cold Spring Harbor Laboratory (CSHL) technical science courses attract talented science students, instructors, and speakers from around the world, and their emphasis on creating a hands-on, immersive experience for a small
Oct 30 2019 | Posted In: 20/20 Blog
Santa Cruz, CA — Members of the Mahajan Lab attended Stanford’s Department of Structural Biology Scientific Retreat held in Santa Cruz at the Chaminade Resort.
May 10 2019 | Posted In: 20/20 Blog
Menlo Park, CA — Gabe Valez, an M.D., Ph.D.
A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay,
Velez, Gabriel, Bassuk Alexander G., Schaefer Kellie A., Brooks Brian, Gakhar Lokesh, Mahajan Maryann, Kahn Philip, Tsang Stephen H., Ferguson Polly J., and Mahajan Vinit B.
, Molecular Case Studies, p.mcs–a002519, (2018)
Structural modeling of a novel SLC38A8 mutation that causes foveal hypoplasia,
Toral, Marcus A., Velez Gabriel, Boudreault Katherine, Schaefer Kellie A., Xu Yu, Saffra Norman, Bassuk Alexander G., Tsang Stephen H., and Mahajan Vinit B.
, Molecular genetics & genomic medicine, Volume 5, p.202–209, (2017)
BESTROPHIN1 mutations cause defective chloride conductance in patient stem cell-derived RPE.,
Moshfegh, Yasmin, Velez Gabriel, Li Yao, Bassuk Alexander G., Mahajan Vinit B., and Tsang Stephen H.
, Hum Mol Genet, 2016 07 01, Volume 25, Issue 13, p.2672-2680, (2016)
Small-angle X-ray scattering of calpain-5 reveals a highly open conformation among calpains,
Gakhar, Lokesh, Bassuk Alexander G., Velez Gabriel, Khan Saif, Yang Jing, Tsang Stephen H., and Mahajan Vinit B.
, Journal of structural biology, Volume 196, p.309–318, (2016)
Structural modeling of a novel CAPN5 mutation that causes uveitis and neovascular retinal detachment.,
Bassuk, Alexander G., Yeh Steven, Wu Shu, Martin Daniel F., Tsang Stephen H., Gakhar Lokesh, and Mahajan Vinit B.
, PLoS One, 2015, Volume 10, Issue 4, p.e0122352, (2015)